Pipette use common problems _ solution _ summary Pipettes, for laboratory personnel, are almost always used in the laboratory, and in the process of use, there will be some problems. First, the common problems of pipette use: Second, the correct pipetting operation: Third, summary: Disposable Long Arm Orange Veterinary Gloves Disposable Orange Veterinary Gloves,Orange Plastic Veterinary Gloves,Disposable Veterinary Gloves,Disposable Plastic Veterinary Gloves Jinan Mucho Commercial Inc. , https://www.muchovet.com
A. Piston/tip leak B. Tip is not suitable for pipette C. Secondary use of tip D. Pipette tip does not touch container wall E. Humidity difference F. No pre-cleaning G. Pipetting rhythm and Unstable time H. Tip depth and pipetting angle are incorrect I. Piston movement is not stable J. Pipette, temperature difference between liquid and room temperature 
1. Pipette the liquid vertically when sucking the liquid 2. Dip the tip at 2-3 mm below the surface of the liquid 3. Perform 3-5 pre-washes during pipetting 4. After pipetting, there should be a gap Suspend 5. Release the liquid at an angle of 30 - 45 ° 6. The pipette should against the inner wall of the receiving container 7. Smooth operation with the thumb
1. Select the correct pipetting mode for different liquid types. 2. Use the pipette manufacturer's original tip 3. Select a small tip (to minimize the amount of air)
4. Keep the pipette, tip and liquid at the same temperature. 5. Use the correct pipetting technique . 6. Reduce the use of manual pipettes and tips, or use a pipette with thermal insulation (mLINE, Proline Plus). )