Study on HPLC Fingerprint of Guan Atractylodes and Analysis of Contents of Atractylenolides I and III [Author] DIAO Ji-hong(Dept.of Pharmacy, The Affiliated Hospital of Yanbian University,Jilin Yanji 133000, China) YANG Shao-qun,LIU Shuang,LIANG Gang,LIANG He,ZHANG Shan-yu(Key Laboratory for Natural Resource Of Changbai Mountain &Functional Molecules,Ministry of Education,Jilin Yanji 133000,China) ã€æœºæž„】 Department of Pharmacy, Affiliated Hospital of Yanbian University; Key Laboratory of Bio-resources and Functional Molecules of Ministry of Education, Changbai Mountain; ã€Abstract】 Objective: To establish a high performance liquid chromatography (HPLC) fingerprint of Guanzhong, and to determine the content of atractylenolide I and III in Guanzhong. METHODS: The chromatographic columns for fingerprinting and content determination were Kromasil C18 (250 mm × 4.6 mm, 5 μm) and OSDhypersil C18 (250 mm × 4.6 mm, 5 μm), respectively. The detection wavelengths were 240 nm and 220 nm, respectively. The mobile phase was water-acetonitrile (gradient). Elution) and methanol-water (60:40, V/V) at a flow rate of 1 mL.min-1 with an injection volume of 10 μL. RESULTS: A total of 9 common fingerprint peaks were calibrated in Guanqing's fingerprints, and the similarity of the 10 batches was >0.9. The injection volumes of atractylenolides I and III showed a good linear relationship with the peak area integral values ​​in the range of 50.1-417.5 ng (r=0.9997) and 49.5-412.5 ng (r=0.9998), respectively. For 101.02% and 97.12%, the RSD was 4.32% and 4.01%, respectively (n is 9). Conclusion: The fingerprints constructed have good precision, repeatability and stability. The method of measuring content is simple, sensitive and reliable, and can be used for quality evaluation and quality control of Guanzhong. More restore Silicone Dressing Wound,Precision Silicone Applicator,Dual Silicone Applicator,Silicone Based Dressing Henan Maidingkang Medical Technology Co.,Ltd , https://www.mdkmedicales.com
[Author] Qi Jihong; Yang Shaoqun; Liu Shuang; Liang Gang; Liang He; Zhang Shanyu;
Abstract: OBJECTIVE:To establish HPLC fingerprint of Atractylodes japonica, and to determine the contents of atractylenolideIand atractylenolide III in A.japonica.METHODS:Fingerprint study and content determination were performed on Kromasil C 18 column(250 mm×4.6 mm, 5 μm And ODS Hypersil C 18 column (250 mm × 4.6 mm, 5 μm); the detection wavelength was set at 240 nm and 220 nm; mobile phase consisted of water-acetonitrile (gradient elution) and methanol-water (60:40, V/V)at the flow rate of 1 mL.min-1.The injection volume was 10 μL.RESULTS:There were 9 common peaks in the fingerprints of A.japonica,and the similarity of 10 batches of samples was higher than 0.9 The linear ranges were 50.1 to 417.5 ng (r = 0.999 7) for atractylenolide Iand 49.5 to 412.5 ng (r = 0.999 8) for atractylenolide III. The average recoveries were 101.02% and 97.12%, and RSDs were 4.32% and 4.01%, Respectively (n=9).CONCLUSION:Established fingerprint has good precision,reproducibility and stability;the method is simple,sen Sitive and reliable.It is suitable for quality evaluation and control of A.japonica.
[Pharmacological action] Atractylenolide I can promote digestion and absorption of the gastrointestinal tract. It is an effective component of the spleen of Atractylodes sinensis. It has anti-inflammatory and anti-tumor effects. It also has the function of regulating gastrointestinal function and promoting absorption of nutrients. Features.