The effects of different sample sizes, different grinding steel ball sizes and different grinding steel balls on the grinding effect of fresh wheat seedlings were tested , and the best experimental scheme for pretreatment of wheat wheat seedlings with TL2020 laboratory small plant pulverizer was explored. DNA provides protection. Material: Fresh wheat, wheat, seedlings Instrument: Mainframe, TL2020 laboratory small plant pulverizer (also known as TL2020 high-flux tissue grinder), the principle is that the horizontal oscillating drive the steel ball in the sample tube to cause high-frequency collision friction with the pipe wall or sample to achieve sample grinding, and the blade The pulverizing principle of the plant pulverizer is more suitable for biological and chemical experiments requiring fine grinding); Accessories: 2ml grinding adapter , 1-8mm diameter grinding steel ball step: 1 ) Sampling: Sampling 20-30 pieces of wheat seedlings with a puncher , or cutting the wheat seedlings into small pieces with scissors; 2 ) Put small pieces / small pieces of wheat into a series of 2ml EP tubes (round bottom), and put about 0.1g in some EP tubes . Put about 1g in some EP tubes . Wherein 0.1g sample tubes were placed in a steel ball diameter 4-8mm (4mm2-3 pieces / tube, 5mm1 pieces / tube, 8mm1 pieces / tube); and 1g sample tube 4mm steel is 1-3 pieces Ball (at this time) 5mm diameter steel ball will cause crowding inside the tube, which will inevitably affect the grinding effect, so do not try); 3) The above EP tubes simultaneously into TL2020 small laboratory mill plant adaptation 2ml polishing holder, and then fixed to the adapter frame of the rocker mills; 4 ) Set the grinding time of TL2020 to 5 minutes and the grinding frequency to 1800 rpm ( 1800 times / min). result: Figure 1: An excessive amount of the sample (> 0.15g), regardless into 1, 2, 3 or 4mm diameter steel balls, the polishing effect is not ideal, there are more granular structure. Figure 2 : When the sample volume of each test tube is " 0.1g or so, no matter whether one 8mm diameter steel ball or one diameter 5mm steel ball or 2-3 diameter 4mm steel balls are placed, the wheat seed sample can be ground into fine. Mud. in conclusion: The amount (weight) of the sample in the grinding tube is critical to the grinding result, and the size and number of the grinding beads are not the most important factors for the grinding result, but the grinding time of the large grinding beads is shorter and smaller than that of the small grinding beads. The finer the fineness of the grinding beads. The sample volume must not be greater than 1/3 of the tube . Attached: 0.1g wheat wheat seedlings, the actual weighing, For reference Keywords: plant shredder, small plant shredder, laboratory small plant crush 2019-Ncov Igm/Igg Antibody Test Kit (Colloidal Gold Method),2019-Ncov Neutralizing Antibody Test Kit (Colloidal Gold Method),2019-Ncov S-Rbd Neutralizing Antibody Test Kit (Colloidal Gold Method),2019-Ncov Neutralizing Antibody Test Kit (Fluorescent Immun Guangdong Hecin Scientific, Inc. , https://www.hecinscientific.com
Test method for grinding fresh wheat seedlings by TL2020 laboratory small plant grinder
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